Myeloperoxidase as an Indicator of Oxidative Stress in Metabolic Syndrome

pp. 538-542

Abstract

Background:

Increased myeloperoxidase (MPO) activity would be the link between the rise of the inflammatory response and oxidative stress (OS) in metabolic syndrome (MS).

Objective:

The aim of this study was to determine the enzymatic activity of MPO associated with OS in animals with MS and establish their relationship with probable cardiovascular injury. 

Methods:

Male Wistar rats were divided into two groups: Group A, control (n=12) and Group B, induced MS (n=12). Metabolic syndrome was produced by 6-week administration of 10% fructose diluted in the drinking water. Insulin (mU/ml), glucose (mg/dl), lipid panel (md/dl), HOMA (homeostatic model assessment), MPO (IU/ml) and superoxide dimutase (SOD) activity (U/ml) were measured. Light microscopy was used for the histological study of the heart and thoracic aorta. 

Results:

Group B showed significantly increased levels of plasma glucose (176±17.3 mg/dl), insulin (29.5 ± 4.52μU/ml), HOMA (11±1.3), total cholesterol (133 ± 9.6 mg/dl) and triglycerides (75 ± 12.9 mg/dl) compared with Group A: plasma glucose (115 ±1.1mg/dl), insulin (4±0.82 μU/ml), HOMA (3±0.38), total cholesterol (69.7±1.6 mg/dl), and triglycerides (46.2 ± 6 mg/dl), (p<0.001 for all variables). A significant decrease in HDL (28.3 ± 1.14 mg/dl) in Group B vs Group A (61 ± 1.01 mg.dl) (p<0.001) validated the experimental MS model. Myeloperoxidase activity increased significantly in Group B (181.3 ± 15.7 UI/ml) vs Group A (116.07±4.2 UI/ml) (p<0,001). A similar behavior was seen with SOD antioxidant activity in Group B (181 ± 6 U/ml) vs. Group A (116.07 ± 4.2 UI/ml) (p<0.01). Light microscopy of the heart and thoracic aorta revealed histopathological change in animals with induced MS. 

Conclusion:

Increased MPO and SOD in Group B would indicate the presence of OS in MS, with consequences at the vascular level.